Safety and immunogenicity of Ad5-nCoV immunization after three-dose priming with inactivated SARS-CoV-2 vaccine in Chinese adults

Abstract Data on the safety and immunity of a heterologous booster (fourth dose) after three-doses of inactivated SARS-CoV-2 vaccine in Chinese adults are limited. We evaluate the safety and immunogenicity of Ad5-nCoV in a randomized, double-blind, parallel-controlled phase 4 clinical trial in Zhejiang, China (NCT05373030). Participants aged 18–80 years (100 per group), administered three doses… Continue reading Safety and immunogenicity of Ad5-nCoV immunization after three-dose priming with inactivated SARS-CoV-2 vaccine in Chinese adults

Phage tailspike modularity and horizontal gene transfer reveals specificity towards E. coli O-antigen serogroups

Arkin AP, Cottingham RW, Henry CS, Harris NL, Stevens RL, Maslov S, Dehal P, Ware D, Perez F, Canon S, Sneddon MW, Henderson ML, Riehl WJ, Murphy-Olson D, Chan SY, Kamimura RT, Kumari S, Drake MM, Brettin TS, Yu D. KBase: the United States department of energy systems biology knowledgebase. Nat Biotechnol. 2018;36(7):566–9. https://doi.org/10.1038/nbt.4163. Article … Continue reading Phage tailspike modularity and horizontal gene transfer reveals specificity towards E. coli O-antigen serogroups

Single-cell sequencing on CD8+ TILs revealed the nature of exhausted T cells recognizing neoantigen and cancer/testis antigen in non-small cell lung cancer

Background CD8+tumor infiltrating lymphocytes (TILs) are often observed in non-small cell lung cancers (NSCLC). However, the characteristics of CD8+ TILs, especially T-cell populations specific for tumor antigens, remain poorly understood. Methods High throughput single-cell RNA sequencing and single-cell T-cell receptor (TCR) sequencing were performed on CD8+ TILs from three surgically-resected lung cancer specimens. Dimensional reduction… Continue reading Single-cell sequencing on CD8+ TILs revealed the nature of exhausted T cells recognizing neoantigen and cancer/testis antigen in non-small cell lung cancer

Prospective, randomized, double-blind phase 2B trial of the TLPO and TLPLDC vaccines to prevent recurrence of resected stage III/IV melanoma: a prespecified 36-month analysis

The TLPLDC vaccine is made by ex vivo priming matured autologous dendritic cells with yeast cell wall particles (YCWPs) loaded with tumor lysate, particle only (TLPO) vaccine . Patients receiving the TLPO vaccine were further divided by their method of DC harvest (with or without G-CSF pretreatment), this was not randomized . Safety was assessed… Continue reading Prospective, randomized, double-blind phase 2B trial of the TLPO and TLPLDC vaccines to prevent recurrence of resected stage III/IV melanoma: a prespecified 36-month analysis

Hallmarks of CD8+ T cell dysfunction are established within hours of tumor antigen encounter before cell division

Abstract Tumor-specific CD8+ T cells (TST) in patients with cancer are dysfunctional and unable to halt cancer progression. TST dysfunction, also known as exhaustion, is thought to be driven by chronic T cell antigen receptor (TCR) stimulation over days to weeks. However, we know little about the interplay between CD8+ T cell function, cell division… Continue reading Hallmarks of CD8+ T cell dysfunction are established within hours of tumor antigen encounter before cell division

Sabin polio virus protein 1 (VP1) evolution in patients with acute flaccid paralysis from 2010 to 2016 in Uganda

It is a retrospective cross-sectional study designed to investigate mutations that are common in patients of acute flaccid paralysis. The permission to undertake this study was obtained from the Ministry of Health, Uganda, the owner of the specimens and the Uganda Virus Research Institute, Research and Ethics Committee approved the study. Archived poliovirus isolates from… Continue reading Sabin polio virus protein 1 (VP1) evolution in patients with acute flaccid paralysis from 2010 to 2016 in Uganda

The key to battling a pathogen hides in its genome

The availability, speed and effectiveness of genomic sequencing increased dramatically during the pandemic . Biologists argue that building on this momentum is critical in society’s response to future pandemics . It requires significant collaboration and investment now, before the next pathogen is threatening society .

Patient-reported outcomes in CD30-directed CAR-T cells against relapsed/refractory CD30+ lymphomas

Chimeric antigen receptor (CAR)-T cells targeting CD30 have demonstrated high response rates with durable remissions observed in a subset of patients with relapsed/refractory CD30+ hematologic malignancies, particularly classical Hodgkin lymphoma. This therapy has low rates of toxicity including cytokine release syndrome with no neurotoxicity observed in our phase 2 study. We collected patient-reported outcomes (PROs)… Continue reading Patient-reported outcomes in CD30-directed CAR-T cells against relapsed/refractory CD30+ lymphomas

Valproic acid increases CAR T cell cytotoxicity against acute myeloid leukemia

Only limited efficacy of chimeric antigen receptor T (CAR-T) cell therapy is observed in acute myeloid leukaemia (AML) patients . CD123 and CLL-1 were highly expressed on AML cells . The proportions of T cell subsets and NK cells involved in anti-tumour processes in patients significantly decreased when compared with healthy donors . A possible… Continue reading Valproic acid increases CAR T cell cytotoxicity against acute myeloid leukemia