Tag: Laboratory Biochemistry

Targeted release of a bispecific fusion protein SIRP{alpha}/Siglec-10 by oncolytic adenovirus reinvigorates tumor-associated macrophages to improve therapeutic outcomes in solid tumors

Background The pleiotropic roles of tumor-associated macrophages (TAMs) render them attractive targets in antitumor drug development. CD47/SIRPα (signal regulatory protein alpha) and CD24/Siglec-10 (sialic acid-binding immunoglobulin-like lectin 10) signaling pathways have been found to suppress macrophage phagocytosis of malignant cells. Systemic blockade of CD47/SIRPα has shown severe side effects. Intratumoral delivery of a CD47 inhibitor… Continue reading Targeted release of a bispecific fusion protein SIRP{alpha}/Siglec-10 by oncolytic adenovirus reinvigorates tumor-associated macrophages to improve therapeutic outcomes in solid tumors

[Newsdesk] Research in brief

No specific antiviral therapies currently exist for hepatitis E virus (HEV) infection. Using flow cytometry and X-ray crystallography, researchers isolated and characterised the antibodies produced in convalescent patients 1 year after clearing acute HEV infection. They noted glycan-sensitive broadly neutralising antibodies that interacted with the non-glycosylated form of the viral capsid protein pORF2. The antibodies… Continue reading [Newsdesk] Research in brief

The Electrically Silent Kv5.1 Subunit Forms Heteromeric Kv2 Channels in Cortical Neurons and Confers Distinct Functional Properties

Voltage-gated K+ channels of the Kv2 family are highly expressed in brain and play dual roles in regulating neuronal excitability and in organizing endoplasmic reticulum-plasma membrane (ER-PM) junctions. Studies in heterologous cells suggest that Kv2.1 and Kv2.2 co-assemble with “electrically silent” KvS subunits to form heterotetrameric channels with distinct biophysical properties, but the prevalence and… Continue reading The Electrically Silent Kv5.1 Subunit Forms Heteromeric Kv2 Channels in Cortical Neurons and Confers Distinct Functional Properties

P-02.02 The influence of fecal supernatants from melanoma patients on CAR T cell cytotoxicity and immune modulation

Background Immune checkpoint inhibitors (ICI) have revolutionized melanoma treatment, yet their efficacy is influenced by factors such as the tumor microenvironment and the host microbiome. Emerging evidence suggests that the microbiome can modulate immune response, though the exact mechanisms remain unclear. This study investigates the impact of fecal supernatants from melanoma patients on chimeric antigen… Continue reading P-02.02 The influence of fecal supernatants from melanoma patients on CAR T cell cytotoxicity and immune modulation

P-01.14 Intracellular and extracellular biochemical functions of V-domain Ig-containing suppressor of T cell activation are regulated by crucial factors of tumour microenvironment

Background V-domain Ig-containing suppressor of T cell activation (VISTA) is a unique multifunctional protein displaying suppressive activities towards anti-cancer immunity. VISTA shows both receptor and ligand activities.1 2 However, the mechanisms of regulation of VISTA activity and functions by factors of tumour microenvironment (TME) remain unclear and understanding these processes is required to develop successful… Continue reading P-01.14 Intracellular and extracellular biochemical functions of V-domain Ig-containing suppressor of T cell activation are regulated by crucial factors of tumour microenvironment

P-02.05 GDF-15 neutralization enhances T cell infiltration in solid tumor models

Background Growth Differentiation Factor 15 (GDF-15) has emerged as a critical immunosuppressive factor in the tumor microenvironment, shown to inhibit endothelial adhesion of leukocytes and limiting T cell infiltration into tumors.1 2 Both chimeric antigen receptor (CAR) T cell therapies and bispecific T-cell engagers have shown remarkable success in hematological malignancies; however, their efficacy in… Continue reading P-02.05 GDF-15 neutralization enhances T cell infiltration in solid tumor models

P-02.06 Chimeric VSV-NDV mediates a multifaceted immune response in solid cancers which is enhanced by expression of high-affinity soluble PD-1

Background For many solid tumor indications, immune-oncology approaches offer little therapeutic benefit due to underlying immune-suppression. Oncolytic virotherapy (OV) can provide the missing piece to the cancer immunotherapy puzzle reprogramming the tumor microenvironment (TME) towards immune stimulation. We have developed a novel chimeric OV platform based on the vesicular stomatitis virus (VSV) backbone and expressing… Continue reading P-02.06 Chimeric VSV-NDV mediates a multifaceted immune response in solid cancers which is enhanced by expression of high-affinity soluble PD-1

P-03.03 Granulopoiesis and thrombopoiesis aberrantly synergize in cancer to promote tumor progression

Background Myeloid cells acquire tumor-supporting properties in cancer. Clinical strategies targeting this pathological process, however, are not established since the underlying mechanisms remain ill-defined. This study aimed at investigating tumor-elicited effects on bone marrow thrombopoiesis and granulopoiesis, thus uncovering the occurence of an aberrant, pro-tumorigenic interplay of these fundamental biological processes in cancer. Materials and… Continue reading P-03.03 Granulopoiesis and thrombopoiesis aberrantly synergize in cancer to promote tumor progression