Abstract Generating effective live vaccines from intact viruses remains challenging owing to considerations of safety and immunogenicity. Approaches that can be applied in a systematic manner are needed. Here we created a library of live attenuated influenza vaccines by using diverse cellular E3 ubiquitin ligases to generate proteolysis-targeting (PROTAR) influenza A viruses. PROTAR viruses were… Continue reading Proteolysis-targeting influenza vaccine strains induce broad-spectrum immunity and in vivo protection
Tag: Influenza
PROTAR Vaccine 2.0 generates influenza vaccines by degrading multiple viral proteins
Abstract Manipulating viral protein stability using the cellular ubiquitin-proteasome system (UPS) represents a promising approach for developing live-attenuated vaccines. The first-generation proteolysis-targeting (PROTAR) vaccine had limitations, as it incorporates proteasome-targeting degrons (PTDs) at only the terminal ends of viral proteins, potentially restricting its broad application. Here we developed the next-generation PROTAR vaccine approach, referred to… Continue reading PROTAR Vaccine 2.0 generates influenza vaccines by degrading multiple viral proteins
G-quadruplex-forming small RNA inhibits coronavirus and influenza A virus replication
Abstract Future pandemic threats may be caused by novel coronaviruses and influenza A viruses. Here we show that when directly added to a cell culture, 12mer guanine RNA (G12) and its phosphorothioate-linked derivatives (G12(S)), rapidly entered cytoplasm and suppressed the propagation of human coronaviruses and influenza A viruses to between 1/100 and nearly 1/1000 of normal… Continue reading G-quadruplex-forming small RNA inhibits coronavirus and influenza A virus replication
The threat of avian influenza H5N1 looms over global biodiversity
The highly pathogenic avian influenza H5N1 is an emerging and unexpected threat to many wild animal species, which has implications for ecological processes, ecosystem services and conservation of threatened species. International collaboration and information-sharing is essential for surveillance, early diagnosis and the provision of financial and technical instruments to enable worldwide actions. Download PDF As… Continue reading The threat of avian influenza H5N1 looms over global biodiversity
TWiV 1183: More than 2024 viruses
TWiV reviews some of their favorite virology stories from 2024. Hosts: Vincent Racaniello, Rich Condit, Kathy Spindler, and Brianne Barker Click arrow to playDownload TWiV 1183 (66 MB .mp3, 111 min) Subscribe (free): Apple Podcasts, RSS, email Become a patron of TWiV! Links for this episode Support science education at MicrobeTV ASV 2025 SARS-CoV-2 origins (TWiV Special) TWiV 1163: Hepadnaviridae in the… Continue reading TWiV 1183: More than 2024 viruses
TWiV 1182: Clinical update with Dr. Daniel Griffin
In his weekly clinical update, Dr. Griffin discusses how vaccination and vaccine hesitancy affects public health and disease spread in terms of mpox, the first human death from H5N1 in US, why one should not feed their pets raw pet food and the metapneumonia outbreak in China before reviewing the recent statistics on RSV, influenza… Continue reading TWiV 1182: Clinical update with Dr. Daniel Griffin
Modulation of cytokeratin and cytokine/chemokine expression following influenza virus infection of differentiated human tonsillar epithelial cells
New molecule shows promise in protecting mice from influenza
Researchers at the University of Gothenburg, Sweden, in collaboration with colleagues in China, have discovered an antibody-like molecule that can protect mice from various influenza viruses. The findings could pave the way for new treatments and the development of broader influenza vaccines. We have identified a small molecule that binds to the virus’s surface protein… Continue reading New molecule shows promise in protecting mice from influenza
Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
Abstract Influenza remains a persistent global health challenge, largely due to the virus’ continuous antigenic drift and occasional shift, which impede the development of a universal vaccine. To address this, the identification of broadly neutralizing antibodies and their epitopes is crucial. Nanobodies, with their unique characteristics and binding capacity, offer a promising avenue to identify such… Continue reading Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
β-Glucan reprograms neutrophils to promote disease tolerance against influenza A virus
Abstract Disease tolerance is an evolutionarily conserved host defense strategy that preserves tissue integrity and physiology without affecting pathogen load. Unlike host resistance, the mechanisms underlying disease tolerance remain poorly understood. In the present study, we investigated whether an adjuvant (β-glucan) can reprogram innate immunity to provide protection against influenza A virus (IAV) infection. β-Glucan… Continue reading β-Glucan reprograms neutrophils to promote disease tolerance against influenza A virus