Sequence-function space of radical SAM cyclophane synthases reveal conserved active site residues that influence substrate specificity

Radical SAM cyclophane synthases catalyze C–C, C–N, and C–O crosslinking reactions in the biosynthesis of bioactive peptide natural products. Here, we studied an uncharacterized rSAM enzyme, HtkB from Pandoraea sp., and found this enzyme to catalyze the formation of a HisC2-to-LysCβ crosslink. We used a combination of ColabFold and mutagenesis studies to show that residues… Continue reading Sequence-function space of radical SAM cyclophane synthases reveal conserved active site residues that influence substrate specificity

GluN3A and Excitatory Glycine Receptors in the Adult Hippocampus

The GluN3A subunit of N-methyl-D-aspartate receptors (NMDARs) plays an established role in synapse development, but its contribution to neural circuits in the adult brain is less clear. Recent work has demonstrated that in select cell populations, GluN3A assembles with GluN1 to form GluN1/GluN3A receptors that are insensitive to glutamate and instead serve as functional excitatory… Continue reading GluN3A and Excitatory Glycine Receptors in the Adult Hippocampus

[ASAP] Ancillary Ligand Steric Effects and Cyclometalating Ligand Substituents Control Excited-State Decay Kinetics in Red-Phosphorescent Platinum Complexes

AI SummaryThis title suggests that the research focuses on how steric effects of ancillary ligands and substituents of cyclometalating ligands can impact the kinetics of excited-state decay in red-phosphorescent platinum complexes. This study likely explores how different ligand structures influence the efficiency of phosphorescent materials, which is crucial for applications in fields such as organic… Continue reading [ASAP] Ancillary Ligand Steric Effects and Cyclometalating Ligand Substituents Control Excited-State Decay Kinetics in Red-Phosphorescent Platinum Complexes

Replication kinetics of pathogenic Eurasian orthohantaviruses in human mesangial cells

AI SummaryThis article explores the replication kinetics of pathogenic Eurasian orthohantaviruses in human mesangial cells. Eurasian orthohantaviruses are known to cause hemorrhagic fever with renal syndrome (HFRS), which is characterized by acute kidney injury (AKI). The study aims to understand how these viruses replicate in human cells, potentially shedding light on the mechanisms behind HFRS… Continue reading Replication kinetics of pathogenic Eurasian orthohantaviruses in human mesangial cells