Tag: Enzyme Kinetics and Mechanisms

Sequence-function space of radical SAM cyclophane synthases reveal conserved active site residues that influence substrate specificity

Radical SAM cyclophane synthases catalyze C–C, C–N, and C–O crosslinking reactions in the biosynthesis of bioactive peptide natural products. Here, we studied an uncharacterized rSAM enzyme, HtkB from Pandoraea sp., and found this enzyme to catalyze the formation of a HisC2-to-LysCβ crosslink. We used a combination of ColabFold and mutagenesis studies to show that residues… Continue reading Sequence-function space of radical SAM cyclophane synthases reveal conserved active site residues that influence substrate specificity

GluN3A and Excitatory Glycine Receptors in the Adult Hippocampus

The GluN3A subunit of N-methyl-D-aspartate receptors (NMDARs) plays an established role in synapse development, but its contribution to neural circuits in the adult brain is less clear. Recent work has demonstrated that in select cell populations, GluN3A assembles with GluN1 to form GluN1/GluN3A receptors that are insensitive to glutamate and instead serve as functional excitatory… Continue reading GluN3A and Excitatory Glycine Receptors in the Adult Hippocampus