Cyanobacteria are widespread, photosynthetic, gram-negative bacteria that generate numerous bioactive secondary metabolites via complex biosynthetic enzymatic machinery. The model cyanobacterium Picosynechococcus sp. strain PCC 7002, hereafter referred to as PCC 7002, contains a type I polyketide synthase (PKS), termed olefin synthase (OlsWT), that synthesizes 1-nonadecene and 1,14-nonadecadiene: α-olefins that are important for growth at low… Continue reading Uncovering the substrate of olefin synthase loading domains in cyanobacteria Picosynechococcus sp. strain PCC 7002
Tag: Enzyme Kinetics and Mechanisms
Targeted inhibition of Aurora kinase A promotes immune checkpoint inhibition efficacy in human papillomavirus-driven cancers
Background Human papillomavirus (HPV)-driven cancers include head and neck squamous cell carcinoma and cervical cancer and represent approximately 5% of all cancer cases worldwide. Standard-of-care chemotherapy, radiotherapy, and immune checkpoint inhibitors (ICIs) are associated with adverse effects and limited responses in patients with HPV-driven cancers. The integration of targeted therapies with ICIs may improve outcomes.… Continue reading Targeted inhibition of Aurora kinase A promotes immune checkpoint inhibition efficacy in human papillomavirus-driven cancers
Routes to molecular glue degrader discovery
Glucokinase: from allosteric glucose sensing to disease variants
Interactions between Arousal State and CO2 Determine the Activity of Central Chemoreceptor Neurons That Drive Breathing
The homeostatic regulation of pulmonary ventilation, and ultimately arterial PCO2, depends on interactions between respiratory chemoreflexes and arousal state. The ventilatory response to CO2 is triggered by neurons in the retrotrapezoid nucleus (RTN) that function as sensors of central pH, which can be identified in adulthood by the expression of Phox2b and neuromedin B. Here,… Continue reading Interactions between Arousal State and CO2 Determine the Activity of Central Chemoreceptor Neurons That Drive Breathing
[ASAP] Au3+-Functionalized Metal–Organic Framework Coordinated Nanotherapeutics for Substrate Self-Supplied Parallel Catalytic and Calcium-Overload-Mediated Therapy of Cancer
ACS Applied Bio MaterialsDOI: 10.1021/acsabm.4c01423
Unlocking the catalytic precision of ligand-controlled enzymatic halogenation
[ASAP] Probing the Histamine H1 Receptor Binding Site to Explore Ligand Binding Kinetics
An Expanded Substrate Scope for Cross-Chiral Ligation Enables Efficient Synthesis of Long L-RNAs
Despite the growing interest in mirror-image L-oligonucleotides, both as a robust nucleic acid analogue and as an artificial genetic polymer, their broader adoption in biochemical research and medicine remains hindered by challenges associated with the synthesis of long sequences, especially for L-RNA. Herein, we present a novel strategy for assembling long L-RNAs via the joining… Continue reading An Expanded Substrate Scope for Cross-Chiral Ligation Enables Efficient Synthesis of Long L-RNAs
An expanded substrate scope for cross-chiral ligation enables efficient synthesis of long L-RNAs
Despite the growing interest in mirror-image L-oligonucleotides, both as a robust nucleic acid analogue and as an artificial genetic polymer, their broader adoption in biochemical research and medicine remains hindered by challenges associated with the synthesis of long sequences, especially for L-RNA. Herein, we present a novel strategy for assembling long L-RNAs via the joining… Continue reading An expanded substrate scope for cross-chiral ligation enables efficient synthesis of long L-RNAs