Background Single-cell adaptive immune receptor (AIR) profiling is a revolutionary approach that allows investigators to combine clonotype repertoire identification with paired-chain information and the phenotype of cells (e.g., cell subtype). Single-cell immune receptor profiling can be performed using a medium-throughput approach (1,000–5,000 cells) using microwell arrays or droplet microfluidics platforms. However, these assays are more… Continue reading 841 A novel 96-well plate-based FACS-sorted single-cell approach allows the identification of immune receptor chain pairing & immunophenotyping of T or B cells
Tag: Diagnostic and Laboratory Virology
781 4-1BB agonist targeted to fibroblast activation protein {alpha} synergizes with radiotherapy to treat murine breast tumor models
Background Ionizing radiation (IR) is a double-edged sword for immunotherapy as it may have both immunosuppressive and immunostimulatory effects. The biological effects of IR on the tumor microenvironment (TME) are a key factor for this balance. Fibroblast activation protein (FAP) is expressed on the surface of cancer associated fibroblasts (CAF) in many cancer types and… Continue reading 781 4-1BB agonist targeted to fibroblast activation protein {alpha} synergizes with radiotherapy to treat murine breast tumor models
419 Utilization of memory NKG2CHi NK cells to target viral antigen peptides demonstrates a novel cellular immunotherapy strategy
Background Natural Killer (NK) cells play a crucial role in innate immune responses against virally infected and transformed cells. The management of human cytomegalovirus (HCMV) infections relies on a subset of NKG2C+ NK cells. The NKG2C/CD94 receptor complex recognize and respond to HCMV-infected cells expressing HLA-E loaded with viral gpUL40 antigen peptide. Upon activation, NKG2C+… Continue reading 419 Utilization of memory NKG2CHi NK cells to target viral antigen peptides demonstrates a novel cellular immunotherapy strategy
346 Reprogramming the tumor microenvironment: endowing T and NK cells with the ability to degrade the extracellular matrix
Background Despite the success of adoptive cell therapy in hematological malignancies, the clinical experience with solid tumors has not been encouraging. The extracellular matrix (ECM) surrounding the tumor presents a significant physical barrier, restricting entry of adoptively transferred cells into the tumor microenvironment (TME). Although T cells expressing the ECM degrading heparanase, have shown preclinical… Continue reading 346 Reprogramming the tumor microenvironment: endowing T and NK cells with the ability to degrade the extracellular matrix
162 TCR repertoire profiling for FFPE RNA and DNA samples from chondrosarcoma reveals the presence of tumor-enriched invariant T cells
Background T-cell receptor (TCR) repertoire profiling is essential to understanding the cancer microenvironment. Assays that can profile TCRs from formalin fixed paraffin embedded (FFPE) samples have been lacking. We collaborated with Cellecta Inc., who have developed the novel multiplex RT-PCR-based DriverMap Adaptive Immune Receptor (AIR) profiling assay that can profile T- or B-cell CDR3 or… Continue reading 162 TCR repertoire profiling for FFPE RNA and DNA samples from chondrosarcoma reveals the presence of tumor-enriched invariant T cells
149 ASPSCR1::TFE3 type-1 vs. type-2 fusions in alveolar soft part sarcoma (ASPS) display a differential tumor immune microenvironment (TIME) influencing response to immune checkpoint inhibitors (ICIs)
Background ASPS is an ultra-rare soft tissue sarcoma characterized by a translocation gene fusion of TFE3 to ASPSCR1, creating a chimeric ASPSCR1::TFE3 fusion protein that functions as an aberrant transcription factor. Depending on where the ASPSCR1 is joined in-frame of TFE3, it generates one of two oncogenic fusion transcripts, type-1 or 2. ASPS has shown… Continue reading 149 ASPSCR1::TFE3 type-1 vs. type-2 fusions in alveolar soft part sarcoma (ASPS) display a differential tumor immune microenvironment (TIME) influencing response to immune checkpoint inhibitors (ICIs)
24 Accelerating antibody discovery for difficult targets using mRNA immunization of wild type or humanized mice and BEACON(R) single cell technology
Background Despite demonstrated efficiency in antibody generation, classical immunization strategies and subsequent hybridoma generation often face strong limitations when it comes to complex targets like GPCRs or tetraspanins. Using WT, KO or Alloy therapeutics ATX-Gx™ humanized transgenic mice, we have developed innovative approaches combining mRNA immunization and Bruker Beacon® single cell screening platform to provide… Continue reading 24 Accelerating antibody discovery for difficult targets using mRNA immunization of wild type or humanized mice and BEACON(R) single cell technology
TWiV 1148: Clinical update with Dr. Daniel Griffin
AI SummaryIn this clinical update, Dr. Daniel Griffin discusses various topics related to the COVID-19 pandemic, including new virus strains from farmed fur animals, comparisons between mRNA and pox vectored vaccines, telehealth management of the disease, prophylactic treatments, and the effects of various treatments on outcomes. He also covers statistics on SARS-CoV-2 infections, viral load… Continue reading TWiV 1148: Clinical update with Dr. Daniel Griffin
ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay
AI SummaryThis article discusses the development of a rapid one-step quadruplex real-time RT-PCR assay called ƩS COVID-19 for diagnosing COVID-19. The assay targets specific genes of SARS-CoV-2 and includes an internal control. It can provide results in 25 minutes with high sensitivity and specificity. Validation showed promising results, making it suitable for rapid diagnosis in… Continue reading ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay
Fetal Zika virus inoculation in macaques revealed control of the fetal viral load during pregnancy
AI SummaryThis study involves inoculating macaques with Zika virus during pregnancy to investigate how the fetal viral load is controlled. The research aims to uncover the mechanisms that lead to brain damage and microcephaly in fetuses infected with Zika virus.Early pregnancy Zika virus (ZIKV) infection is associated with major brain damage in fetuses, leading to… Continue reading Fetal Zika virus inoculation in macaques revealed control of the fetal viral load during pregnancy