Background Despite the success of adoptive cell therapy in hematological malignancies, the clinical experience with solid tumors has not been encouraging. The extracellular matrix (ECM) surrounding the tumor presents a significant physical barrier, restricting entry of adoptively transferred cells into the tumor microenvironment (TME). Although T cells expressing the ECM degrading heparanase, have shown preclinical… Continue reading 346 Reprogramming the tumor microenvironment: endowing T and NK cells with the ability to degrade the extracellular matrix
Tag: Diagnostic and Laboratory Virology
162 TCR repertoire profiling for FFPE RNA and DNA samples from chondrosarcoma reveals the presence of tumor-enriched invariant T cells
Background T-cell receptor (TCR) repertoire profiling is essential to understanding the cancer microenvironment. Assays that can profile TCRs from formalin fixed paraffin embedded (FFPE) samples have been lacking. We collaborated with Cellecta Inc., who have developed the novel multiplex RT-PCR-based DriverMap Adaptive Immune Receptor (AIR) profiling assay that can profile T- or B-cell CDR3 or… Continue reading 162 TCR repertoire profiling for FFPE RNA and DNA samples from chondrosarcoma reveals the presence of tumor-enriched invariant T cells
149 ASPSCR1::TFE3 type-1 vs. type-2 fusions in alveolar soft part sarcoma (ASPS) display a differential tumor immune microenvironment (TIME) influencing response to immune checkpoint inhibitors (ICIs)
Background ASPS is an ultra-rare soft tissue sarcoma characterized by a translocation gene fusion of TFE3 to ASPSCR1, creating a chimeric ASPSCR1::TFE3 fusion protein that functions as an aberrant transcription factor. Depending on where the ASPSCR1 is joined in-frame of TFE3, it generates one of two oncogenic fusion transcripts, type-1 or 2. ASPS has shown… Continue reading 149 ASPSCR1::TFE3 type-1 vs. type-2 fusions in alveolar soft part sarcoma (ASPS) display a differential tumor immune microenvironment (TIME) influencing response to immune checkpoint inhibitors (ICIs)
24 Accelerating antibody discovery for difficult targets using mRNA immunization of wild type or humanized mice and BEACON(R) single cell technology
Background Despite demonstrated efficiency in antibody generation, classical immunization strategies and subsequent hybridoma generation often face strong limitations when it comes to complex targets like GPCRs or tetraspanins. Using WT, KO or Alloy therapeutics ATX-Gx™ humanized transgenic mice, we have developed innovative approaches combining mRNA immunization and Bruker Beacon® single cell screening platform to provide… Continue reading 24 Accelerating antibody discovery for difficult targets using mRNA immunization of wild type or humanized mice and BEACON(R) single cell technology
TWiV 1148: Clinical update with Dr. Daniel Griffin
AI SummaryIn this clinical update, Dr. Daniel Griffin discusses various topics related to the COVID-19 pandemic, including new virus strains from farmed fur animals, comparisons between mRNA and pox vectored vaccines, telehealth management of the disease, prophylactic treatments, and the effects of various treatments on outcomes. He also covers statistics on SARS-CoV-2 infections, viral load… Continue reading TWiV 1148: Clinical update with Dr. Daniel Griffin
ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay
AI SummaryThis article discusses the development of a rapid one-step quadruplex real-time RT-PCR assay called ƩS COVID-19 for diagnosing COVID-19. The assay targets specific genes of SARS-CoV-2 and includes an internal control. It can provide results in 25 minutes with high sensitivity and specificity. Validation showed promising results, making it suitable for rapid diagnosis in… Continue reading ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay
Fetal Zika virus inoculation in macaques revealed control of the fetal viral load during pregnancy
AI SummaryThis study involves inoculating macaques with Zika virus during pregnancy to investigate how the fetal viral load is controlled. The research aims to uncover the mechanisms that lead to brain damage and microcephaly in fetuses infected with Zika virus.Early pregnancy Zika virus (ZIKV) infection is associated with major brain damage in fetuses, leading to… Continue reading Fetal Zika virus inoculation in macaques revealed control of the fetal viral load during pregnancy
Quiescence Enhances Survival during Viral Infection in Caenorhabditis elegans
AI SummaryThe article discusses how quiescence, a state of reduced activity similar to sleep, enhances survival during viral infection in Caenorhabditis elegans. Infection with the Orsay virus affects behavior, highlighting gut-to-nervous system communication. Quiescence during infection has the sleep properties of reduced responsiveness and rapid reversibility. Certain neurons regulate virus-induced quiescence, with a potential role… Continue reading Quiescence Enhances Survival during Viral Infection in Caenorhabditis elegans
Resistance to protease inhibitors among persons living with HIV in Ghana: a case for viral load and drug resistance monitoring
Routine viral load and drug resistance testing are well supported in most resource-rich settings and provide valuable benefits in the clinical care of PLWH in these communities. Undoubtedly, there exist financ… Continue reading on BioMed Central
TWiV 1119: Gene editing latent herpes
TWiV reviews viral shedding and antibody responses of men with acute monkeypox virus infection, and gene editing for latent herpes simplex virus infection reduces viral load and shedding. Hosts: Vincent Racaniello, Rich Condit, Kathy Spindler, and Angela Mingarelli Click arrow to playDownload TWiV 1117 (61 MB .mp3, 101 min) Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links… Continue reading TWiV 1119: Gene editing latent herpes