Label-free fluorescence-based chemosensing has been increasingly brought into focus due to its simplicity and high sensitivity for intracellular monitoring of molecules. Currently used methods, such as conventional indicator displacement assays (IDAs), pose limitations related to dissociation upon dilution, random diffusion of the released indicator, and high sensitivity to interference by agents from the ambient cellular… Continue reading Binding affinity-based intracellular drug detection enabled by a unimolecular cucurbit[7]uril-dye conjugate
Tag: Biochemical Pharmacology
Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1
AI SummarySynaptic vesicle (SV) endocytosis is an important process for maintaining neurotransmission. The absence of synaptotagmin-11 (Syt11) accelerates SV endocytosis and leads to abnormal distribution of synaptic proteins. Syt11 interacts with endophilin A1 (EndoA1), inhibiting its function and ensuring the precision of protein retrieval during SV endocytosis. Synaptic vesicle (SV) endocytosis is a critical and… Continue reading Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1
Structure and dynamics of differential ligand binding in the human ρ-type GABAA receptor
Cowgill et al. report apo, inhibited, and desensitized structures of the human ρ1 GABAA receptor. These structures, combined with supporting simulations and electrophysiology experiments, reveal a more compact neurotransmitter-binding site that defines the unique function and pharmacology of ρ-type GABAA receptors relative to canonical synaptic GABAA receptors.
Atlas of human brain blood vessels highlights changes in Alzheimer’s disease
AI SummaryScientists from MIT’s Computer Science and Artificial Intelligence Laboratory (CSAIL), The Picower Institute for Learning and Memory, and The Broad Institute of MIT and Harvard have created a molecular atlas of human brain vasculature and its changes in Alzheimer’s disease (AD) across six brain regions. The researchers characterized over 22,514 vascular cells from different… Continue reading Atlas of human brain blood vessels highlights changes in Alzheimer’s disease