Category: Biochemistry

Zeolites as equilibrium-shifting agents in shuttle catalysis

Abstract The catalytic shuttling of functional moieties has emerged as a promising strategy to substitute and diversify traditional hydrofunctionalization technologies. However, these reactions are reversible due to their isodesmic nature, which limits their applicability to a select array of donor and acceptor molecules, and poses substantial challenges with regard to atom economy and practicality. Here… Continue reading Zeolites as equilibrium-shifting agents in shuttle catalysis

Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression

Abstract The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. However, generating these models is time- and resource-intensive. Here we describe a model system, SELective Expression and Controlled Transduction In Vivo (SELECTIV), that enables efficient and specific expression of transgenes… Continue reading Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression

A flexible system for tissue-specific gene expression in mice using adeno-associated virus

Cell type-specific transgene expression in mice has broad utility in biomedical research. We developed a versatile system for in vivo transgene delivery using adeno-associated virus (AAV). Efficient and tissue-specific transgene expression is achieved by regulating the expression of the gene encoding the AAV receptor, thereby precisely targeting AAV to the cell type of interest. This… Continue reading A flexible system for tissue-specific gene expression in mice using adeno-associated virus