Directed protein evolution for generating antibodies with improved binding affinity or stability requires exploration of a vast space of possible mutations. Experimental high-throughput antibody engineering methods screen thousands to millions of variants using techniques like phage display or cell surface display. This imposes a heavy experimental burden. Computational methods provide a structure-guided rationale for selecting… Continue reading Protein language models guide directed antibody evolution
Category: Biochemistry
Assembly-free phylogenetic trees
The rise of genomic big data has transformed many aspects of the decades-long research on phylogenetic relationship inference. To maximize information extracted from sequenced biomolecules, computational pipelines have been developed that include steps such as genome assembly, annotation and orthology identification, all of which are equipped with dedicated algorithms. Despite being remarkably powerful, these complex… Continue reading Assembly-free phylogenetic trees
Tracing lymphocyte development
The function of responding T or B cells is strongly influenced by their cell surface lymphocyte receptor. Although methods for multiomic analysis have enabled the study of lymphocytes at unprecedented resolution, there still remain some gaps in our interpretation of transcriptomic data in the context of the adaptive immune receptor repertoire (AIRR). To address this,… Continue reading Tracing lymphocyte development
Mass spectrometry uncovers actions of protein ‘glues’
Chemical Science (2023). DOI: 10.1039/D3SC01732J”> (A) Illustrates the limited understanding of MG1 induced complex stabilization. (B) Schematic representation of MG1 ligation. (C) Enlarged view of the 14-3-3/Pin1/MG1 interface . (D) MG1 covalently bound to Lys122 of 14-3-3 by aldimine bonding. Credit: Chemical Science (2023). DOI: 10.1039/D3SC01732J A screening technique commonly used in drug discovery can… Continue reading Mass spectrometry uncovers actions of protein ‘glues’
[ASAP] In Vivo Laser-Induced Vasoactive Microenvironmental Setting via a Stimuli-Responsive Microstructured Depot
BiomacromoleculesDOI: 10.1021/acs.biomac.3c00125
[ASAP] Deoxyribonucleic Acid-Based Polyvalent Ligand–Receptor Binding for Engineering the Cell Surface with Nanoparticles
BiomacromoleculesDOI: 10.1021/acs.biomac.3c00264
[ASAP] Radioactive and Fluorescent Dual Modality Cysteine Cathepsin B Activity-Based Probe for Cancer Theranostics
The study of chemical pharmacyDOI: 10.1021 / acs. Molpharmaceut. 3c00148.