A flowchart for adequate controls in virus-based monosynaptic tracing experiments identified Cre-independent leakage of the TVA receptor in RΦGT mice

AI Summary

This study discusses the development of a flowchart to ensure adequate controls in virus-based monosynaptic tracing experiments. Specifically, the researchers identified Cre-independent leakage of the TVA receptor in RΦGT mice. They used a modified rabies virus lacking the rabies glycoprotein to trace neuronal connections and found unexpected leakage of the TVA receptor. This finding highlights the importance of rigorous control experiments in viral tracing studies to ensure accurate results.