Researchers have detailed PEPPI-MS, an experimental protocol combining high-resolution protein fractionation using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with the latest mass spectrometry technology to enable highly sensitive analysis of proteoforms, the diverse chemical structures of proteins. This protocol paper is expected to contribute to the development of disease diagnostics based on proteoform information.
PEPPI-MS protocols reveal chemical structure of cellular proteins on a large scale
