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This article discusses the use of tautomer-selective fluorescence spectroscopy to study oxyluciferin anions. The research focuses on understanding the different tautomeric forms of oxyluciferin and their fluorescence properties. This study has implications for studying bioluminescence and could provide insights into the mechanisms of firefly bioluminescence. The term "oxyluciferin" refers to the light-emitting compound found in fireflies, while "fluorescence spectroscopy" is a technique used to study the emission of light by molecules. By studying tautomer-selective fluorescence of oxyluciferin anions, researchers hope to gain a better understanding of the mechanisms behind firefly bioluminescence.