Adoptive cell therapy (ACT) has shown promising results for the treatment of cancer and viral infections . Successful ACT relies on ex vivo expansion of large numbers of desired T-cells with strong cytotoxic capacity and in vivo persistence . Here, in this study, we present a novel technology for ex-vital expansion . artificial antigen-presenting scaffolds (Ag-scaffolds) consisting of a dextran-polysaccharide backbone, decorated with combinations of peptide-Major Histocompatibility Complex (pMHC), cytokines and co-stimulatory molecules .