As a crucial messenger molecule, hydrogen sulfide( H2S ) is essential to normal cell function. However, the development of pathological states such as neurodegenerative diseases, cardiovascular disorders, and cancer is directly correlated with abnormal H2S levels, particularly mitochondrial levels. Therefore, it is essential to monitor fluxes of mitochondrial H2S concentrations in vitro and in vivo with high selectivity and sensitivity. In this regard, we created the first-ever instance of a mitochondria-targeted, H2S-responsive new generation 1, 2-dioxetane-based chemiluminescent probe( MCH ). Compared to traditional fluorophores, chemical luminescent probes have special advantages because they don’t need external light irradiation to emit light. When MCH reacted with H2S with a high selectivity, it displayed an abrupt turn-on response in its luminescence signal. It was used to find H2S activity in a variety of biological systems, including tumor-carrying mice, human serum, and cancerous cells. In order to imaging various analytes in various biological models,
A mitochondria-targeted chemiluminescent probe for detection of hydrogen sulfide in cancer cells, human serum and in vivo
