1. According to an analysis of # TNF-alpha and # IFN-gamma levels, the” weaning reaction” in mouse pups is a” physiological CD4 + T cell response to # microbiota + solid # foods that happens between 2 and 4 weeks after birth. It doesn’t happen in # germfree pups without a microbiota. pic. twitter.com / pSGWXOUBoC— David Usharauli on March 21, 2019(@ 3DiMMUNE )
3. 3. More importantly, though, # microbiota exposure between 2 and 4 weeks after birth, but not later, could help # germfree pups’ T cells regain their physiological “# weaning reaction.” # tolerance # immunologypic. 9KUtk3MT7R on twitter.com— David Usharauli on March 21, 2019 (@ 3DiMMUNE )
5. 5. It’s interesting to note that the presence of gut residing RORgt + FOXP3 + T regulatory cells ( Tregs ) was necessary for the” weaning reaction” in # newborn pups. # tolerancepic. twitter.com / s1nq2HPShK— David Usharauli on March 21, 2019(@ 3DiMMUNE )
Giving # SCFAs to # antibiotic-treated newborn pups could prevent # inflammation later, according to the second correction (# 6 ). It appears that this SCFA effect necessitates # Tregs and # microbiota( it does not function in GF pups ) that gives Treg genes to keep them in# functioning state. pic. Twitter.com / 2ewMoDRobh— David Usharauli on March 21, 2019(@ 3DiMMUNE )
This suggests that SCFAs’ effect is mediated by Tregs and that the” weaning reaction” that is artificially induced with SCFs but lacks proper treg contribution( driven by the microbiota and their antigens ) is not ideal.— David Usharauli on March 21, 2019(@ 3DiMMUNE )